| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Murray, Miranda B (11)
|
MAPFY |
35.61 |
34.94 |
-0.67 |
| 2 |
Bubser, Kelci L (12)
|
MAPAAC |
38.60 |
37.26 |
-1.34 |
| 3 |
Persaud, Hannah (12)
|
MAPFY |
40.01 |
39.36 |
-0.65 |
| 4 |
Quinones, Kaya F (12)
|
MAPFY |
39.06 |
40.16 |
1.10 |
| 5 |
Fleischaker, Leyna S (11)
|
MAPAAC |
42.32 |
42.82 |
0.50 |
| 6 |
Brown, Kyran I (11)
|
MAUN |
44.31 |
44.80 |
0.49 |
| 7 |
Colarusso, Kira R (12)
|
MAPAAC |
|
44.96 |
- |
| 8 |
Emory-Brander, Jenna J (11)
|
MAEAST |
55.79 |
49.53 |
-6.26 |
| 9 |
Dolan, Rachel M (11)
|
MAPAAC |
|
51.36 |
- |
| 10 |
Brown, Yasmin N (12)
|
MASERP |
|
54.59 |
- |
|
Dhareshwar, Arti S (11)
|
MAPAAC |
45.37 |
NS |
- |